REPO-NFκB 报告基因是吉满生物自主研发的用于检测 NFκB 转录活性水平为目的的报告基因。 NFκB 基因在炎症反应、免疫应答和抑制细胞凋亡等方面有着重要的作用。
REPO-NFκB 报告基因是吉满生物自主研发的用于检测 NFκB 转录活性水平为目的的报告基因。 NFκB 基因在炎症反应、免疫应答和抑制细胞凋亡等方面有着重要的作用。
货号 | 规格 | 价格 | 储运 | 加入购物车 |
描述
元件顺序 | NF-κB-Luc Reporter |
表达方式 | 其他 |
荧光 | N/A |
真核抗性 | Neomycin |
工具类型 | 质粒 |
用途 | 报告基因 |
REPOTMNF-κB报告基因主要应用于NF-κB信号通路的研究和基于NF-κB信号通路的药物研究以及癌细胞中NF-KB的调控机制的研究。
pGMNF-ΚB-Lu是吉满生物改造后的哺乳动物真核表达载体,在其多克隆位点插入了多个NFκB结合位点,可以高灵敏度地检测NFκB的激活水平。同时,对载体中预测出的其它转录因子以外的结合位点进行了适当的突变,增加了质粒的转录因子结合特异性。另外,由于质粒体积减小,使得NFκB报告基因质粒更易于转染。
效果验证
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The luciferase reporter construct pGMNF-κB-Lu was cotransfected transiently with pSG5 or GyrB-PKR-K296H into MIN6 cells grown in 24-well plates, using the lipofectamine 2000 reagent according to the manufacturer’s instructions.TRAF2 plays a critical role in PKR protein-binding–induced NF-κB activation and proliferation in MIN6 cells. MIN6 cells were transfected with si-NC, si-TRAF2 or si-TRAF6 before GyrB-PKR-K296H plasmids. After 48 h of gene silencing, cells were treated with coumermycin as indicated. (A) Luciferase activity was measured and shown in fold |
客户文章
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Gu C, Wang F, Zhao Z, et al. Lysophosphatidic acid is associated with atherosclerotic plaque instability by regulating NF-κB dependent matrix metalloproteinase-9 expression via LPA2 in macrophages[J]. Frontiers in Physiology, 2017, 8.
相关产品
GM-022001:NF-κB报告基因慢病毒