最新！一季度客户文献精选集锦来啦
2024年一季度共收集引用Genomeditech Co.,Ltd. (Shangha,China)文章共计95篇，影响因子(IF)近680分。其中包括Cell、Cell Metabolism、Nano Today、Nature communications、Advanced Science影响因子15+以上的文章8篇。
文章所涵盖的产品和服务包含常规质粒构建、慢病毒、腺相关病毒、细胞株构建、活体成像、荧光素酶报告基因，还包含了转染试剂，慢病毒包装试剂盒，siRNA等常用科研产品类试剂。
本篇精选了一季度新发的部分高质量文章，让我们一起追踪最新科研进展！
质粒产品篇
1、中性粒细胞图谱阐明其抗肿瘤过程中的抗原呈递作用
文献标题：Neutrophil profiling illuminates anti-tumor antigen-presenting potency
影响因子：64.5
发表期刊：Cell
发表单位：复旦大学附属中山医院
摘要：中性粒细胞是抵御病原体最丰富和最有效的防御者，在不同癌症类型中发挥相反的功能。然而，由于它们的半衰期较短，探索中性粒细胞如何在癌症中采取特定的命运仍然具有挑战性。在这里，作者从17种癌症类型（来自143例患者的225个样本）中生成并整合了单细胞中性粒细胞转录组。中性粒细胞表现出异常的复杂性，有10种不同的状态，包括炎症、血管生成和抗原呈递。值得注意的是，在大多数癌症中，抗原-呈递程序与良好的生存率相关，并可通过亮氨酸代谢和随后的组蛋白H3K27ac修饰引起。这些中性粒细胞可以进一步引起（neo）抗原特异性和抗原非依赖性T细胞反应。中性粒细胞传递或亮氨酸饮食微调免疫平衡，以增强抗PD-1治疗。总之，这些数据不仅表明了不同癌症间中性粒细胞的差异，而且还提示了治疗机会，如抗原呈递中性粒细胞传递。
吉满助力
Human: dHL-60 cells. 人早幼粒白血病细胞
RFX5 过表达和干扰质粒
2、组蛋白乙酰转移酶6A介导CD4+T细胞糖代谢重编程影响自身免疫疾病
文献标题：Lysine acetyltransferase 6A maintains CD4+ T cell response via epigenetic reprogramming of glucose metabolism in autoimmunity
影响因子：28.9
发表期刊：Cell Metabolism
发表单位：上海市第九人民医院
吉满助力
KAT6A 敲除质粒
原文引用：The CRISPR/Cas9 system was used to delete the genomic DNA of mouse KAT6A ex vivo. sgRNAs targeting mouse KAT6A and nontargeting control (mouse) sgRNA were designed (Table S4), cloned and inserted into a retro-gRNA-eGFP vector. gRNA was synthesized by Genomeditech Corp. (Shanghai, China).
3、Cullin5通过调节肺泡巨噬细胞抗病毒免疫来驱动实验性哮喘恶化
文献标题：Cullin5 drives experimental asthma exacerbations by modulating alveolar macrophage antiviral immunity
影响因子：16.6
发表期刊：Nature communications
发表单位：上海交通大学药学院
吉满助力
Ogt shRNA干扰质粒
原文引用：Mouse Ogt short hairpin RNA (shRNA) vector(pGMLV-SB3) was purchased from Genomeditech. Co., Ltd. (Shanghai,China), and the sequences are shown in Supplementary Table 3.
报告基因产品篇
4、放射治疗诱导的星形胶质细胞衰老促进胶质母细胞瘤的免疫抑制微环境，促进肿瘤再生
文献标题：Radiotherapy-Induced Astrocyte Senescence Promotes an Immunosuppressive Microenvironment in Glioblastoma to Facilitate Tumor Regrowth
影响因子：15.1
发表期刊：Advanced Science
发表单位：浙江大学医学院附属第二医院
吉满助力
Myc-Luc和CXCL1启动子报告基因质粒
原文引用：The Myc-Max responsive luciferase reporter plasmid (Myc-Luc reporter), and CXCL1 promoter luciferase reporter plasmids (pGL3-CXCL1-Wild-type [WT]/DEL1/DEL2) were synthesized by Genomeditech Co., Ltd(Shanghai, China).
5、Maf1 缺失可调节脊柱生成并减轻阿尔茨海默病中的认知障碍
文献标题：Maf1 loss regulates spinogenesis and attenuates cognitive impairment in Alzheimer’s disease
影响因子：14.5
发表期刊：Brain
发表单位：同济大学医学院附属上海东方医院
吉满助力
Maf 过表达质粒和 Grin1启动子报告基因质粒
原文引用：The Maf overexpressed plasmid, the wild-type Grin1 promoter luciferase (Luc) and mutant Grin1 promoter luciferase were constructed by Genomeditech Co. Ltd.
6、YAP 调控的 APIP 促进头颈鳞状细胞癌中的蛋氨酸循环和转移
文献标题：APIP regulated by YAP propels methionine cycle and metastasis in head and neck squamous cell carcinoma
影响因子：9.7
发表期刊：Cancer Letters
发表单位：上海市第九人民医院
吉满助力
APIP启动子报告基因质粒
原文引用：Targeting the APIP promoter region were synthesized by Genomeditech (Shanghai, China).
7、阻断 EGR1/TGF-β1和 CD44s/STAT3交互抑制胃癌腹膜转移
文献标题：Blocking EGR1/TGF-β1 and CD44s/STAT3 Crosstalk Inhibits Peritoneal Metastasis of Gastric Cancer
影响因子：9.2
发表期刊：International Journal of Biological Sciences
发表单位：上海交通大学医学院瑞金医院
吉满助力
TGF-β1启动子报告基因质粒和引物
原文引用：Lentiviruses were constructed with specific primers(forward: 5’-GATCCGCGCAGATCGATTTGA-3’,
reverse: 5’-AATTAAAAAAGCGCAGATCGAT-3’)from Genomeditech (Shanghai, ChinaThe wild-type TGF-β1promoter (WT, -1900 to +100 bp), EGR1 binding site 1mutated TGF-β1 promoter (MT1, -1900 to +100 bp),EGR1 binding site 2 mutated TGF-β1 promoter (MT2,-1900 to +100 bp) and binding sites truncated TGF-β1promoter (MT3, -1900 to -183 bp) were cloned into theluciferase reporter vector pGL3 basic (Genomeditech)
腺相关病毒产品篇
8、慢性皮炎中 20-HETE 介导的 TRPV1 激活通过 MrgprA3+ 神经元驱动异动
文献标题：20-HETE mediated TRPV1 activation drives allokinesis via MrgprA3+ neurons in chronic dermatitis
影响因子：12.4
发表期刊：Theranostics
发表单位：南京中医药大学
吉满助力
hM4Di-mCherry 化学遗传AAV
原文引用：To genetically silence MrgprA3 neurons using the inhibitory, Gi-coupled hM4Di DREADD (designer receptors exclusively activated by designer drugs) receptor, hM4Di-mCherry adeno-associated virus (AAV) (Genomeditech, China) was injected by tail vein (1011 VG in 100 μL) into hemizygous MrgprA3-Cre and MrgprA3.
9、吡那地尔通过抑制分子伴侣介导的钙网蛋白自噬改善心脏微血管缺血再灌注损伤
文献标题：Pinacidil ameliorates cardiac microvascular ischemia-reperfusion injury by inhibiting chaperone-mediated autophagy of calreticulin
影响因子：9.49
发表期刊：Basic Research in Cardiology
发表单位：复旦大学附属中山医院
吉满助力
AAV9-CRT过表达AAV
原文引用：CRT-overexpressing adeno-associated virus serotype 9 (AAV9-CRT) genomic particles were obtained from Genomeditech (Shanghai, China). To construct an endothelial cell-specific CRT overexpression mouse model, 5×1011AAV9-CRT or its negative control (AAV9-NC) genome particles was injected into 6-week-old C57BL/6 J mice via the caudal vein.
10、尼可地尔减轻糖尿病心肌病中心脏微血管铁死亡：线粒体定位的 AMPK-Parkin-ACSL4 信号通路的作用
文献标题：Nicorandil alleviates cardiac microvascular ferroptosis in diabetic cardiomyopathy: Role of the mitochondria-localized AMPK-Parkin-ACSL4 signaling pathway
影响因子：9.3
发表期刊：Pharmacological Research
发表单位：复旦大学附属中山医院
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AAV9-CA-mitoAα1过表达AAV；LV-shParkin干扰慢病毒和LV-mitoACSL4、LV-mitoAα1，LV-CA-mitoAα1过表达慢病毒
原文引用：Adeno-associated virus serotype 9 (AAV9) carrying constitutively active AMPKα1 (amino acid residues from 1 to 312), containing Tie2 promoter, 3 ×Flag tag, and mitochondrial transit peptide (MTP), was defined as AAV9-CA-mitoAα1 and constructed by Genomeditech (Shanghai, China) according to methods described in a previous study.Lentiviruses containing short hairpin RNA targeting Parkin (LV-shParkin) and the negative control (LV-shNC) were constructed by Genomeditech (Shanghai, China). The LV packaging vectors of MTP-fused ACSL4 sequence (LV-mitoACSL4), MTP-fused AMPKα1 sequence (LV-mitoAα1), and MTP-fused CA-AMPKα1 sequence (LV-CA-mitoAα1) were designed by and obtained from Genomeditech (Shanghai, China)
11、丙酸通过调节背根神经节感觉 TRP 通道减轻特应性皮炎小鼠模型的瘙痒
文献标题：Propionate alleviates itch in murine models of atopic dermatitis by modulating sensory TRP channels of dorsal root ganglion
影响因子：9.2
发表期刊：International Journal of Biological Sciences
发表单位：上海交通大学医学院瑞金医院
吉满助力
AAV9-GPR41 干扰AAV
原文引用：The AAV9-GPR41 shRNA and control vectors were produced and purified as previously reported (Genomeditech).
慢病毒产品篇
12、TET2介导的cGAS-STING通路在肝癌细胞和内皮细胞间交互调控血管正常化和免疫治疗
文献标题：TET2-mediated tumor cGAS triggers endothelial STING activation to regulate vasculature remodeling and anti-tumor immunity in liver cancer
影响因子：16.6
发表期刊：Nature Communications
发表单位：海军军医大学
吉满助力
Tet2干扰慢病毒
原文引用：The Lentivirus-mediated shRNA expressing vectors targeting murine Tet2 (target sequence: 5′-GCTCTAAATGATGTAGCTTTG-3′) were purchased from Genomeditech (Shanghai, China) .
13、SOCS5-RBMX 刺激 SREBP1 介导的脂肪生成，促进伴有 HBV 相关肝硬化的脂肪变性 HCC 的转移
文献标题：SOCS5-RBMX stimulates SREBP1-mediated lipogenesis to promote metastasis in steatotic HCC with HBV-related cirrhosis
影响因子：7.9
发表期刊：npj Precision Oncology
发表单位：青岛大学附属医院
吉满助力
SOCS5过表达和干扰慢病毒
原文引用：Lentivirus of short hairpin RNAs (shRNAs) targeting SOCS5, SOCS5 overexpression lentivirus and the control lentivirus were purchased from Genomeditech(Shanghai, China).
14、山奈酚通过 circ_0000345介导的 JMJD2C/β-catenin信号通路抑制大肠癌转移
文献标题：Kaempferol inhibits colorectal cancer metastasis through circ_0000345 mediated JMJD2C/β-catenin signalling pathway
影响因子：7.9
发表期刊：Phytomedicine
发表单位：南京中医药大学附属苏州中医医院
吉满助力
circ_0000345干扰慢病毒和对照
原文引用：Genomeditech (Shanghai, China) supplied the lenti-short hairpin-circ0000345 (sh-circ_0000345) stable lentiviral vector for circ_0000345 knockdown, along with the short-hairpin negative control (sh-NC).
15、ChemR23 信号通过抑制 NLRP3 炎性体介导的缺血性脑卒中神经元焦亡来改善脑损伤
文献标题：ChemR23 signaling ameliorates brain injury via inhibiting NLRP3 inflammasome-mediated neuronal pyroptosis in ischemic stroke
影响因子：7.4
发表期刊：Journal of Translational Medicine
发表单位：上海市第六人民医院
吉满助力
ChemR23过表达慢病毒和对照
原文引用：A lentiviral vector overexpressing ChemR23 (OE) and a negative control vector (Lv-con) were purchased from Genomeditech (Shanghai, China).
16、脂肪团和肥胖相关蛋白通过 FYN/Drp1轴抑制神经元铁浓缩，减轻脑缺血再灌注损伤
文献标题：Fat mass and obesity associated protein inhibits neuronal ferroptosis via the FYN/Drp1 axis and alleviate cerebral ischemia/reperfusion injury
影响因子：5.5
发表期刊：CNS Neuroscience & Therapeutics
发表单位：湖南省人民医院
吉满助力
慢病毒和polybrene
原文引用：Lentivirus particles were purchased from Genomeditech (Shanghai, China).The next day, the prepared lentivirus particles were utilized for 24-h cell infection and 5 μg/mL Polybrene (GM-040901, Genomeditech).
siRNA系列
17、飞秒激光对 Bmp 受体的无视蛋白激活
文献标题：Opsin-Free Activation of Bmp Receptors by a Femtosecond Laser
影响因子：16.6
发表期刊：Nature communications
发表单位：上海交通大学生物医学工程学院
吉满助力
BMP siRNA
原文引用：Synthetic small interference RNA (siRNA) sequences targeting BMP receptors type Ia was synthesized and purchased from Genomeditech, Shanghai. siRNA with a nontargeting sequence (siNC) was used as a negative control.
18、黑色素瘤来源的外泌体通过巨噬细胞中的 IRF7/I-IFN 轴放大放疗诱导的远隔效应
文献标题：Melanoma Derived Exosomes Amplify Radiotherapy Induced Abscopal Effect via IRF7/I-IFN Axis in Macrophages
影响因子：15.1
发表期刊：Advanced Science
发表单位：复旦大学附属中山医院
吉满助力
IRF7 siRNA和miR-872-3p mimics
原文引用：small interfering RNA targeting IRF7 with the sequence CCAGAUGCGUGUUCCUGUA was designed and synthesized by Genomeditech (Shanghai,China). miR-872-3p mimics and control plasmids were purchased fromGenomeditech (Shanghai, China)
19、骨细胞线粒体通过STING通路抑制癌症转移和发展
文献标题：Osteocyte mitochondria inhibit tumor development via STING-dependent antitumor immunity
影响因子：13.6
发表期刊：Science Advances
发表单位：浙江大学医学院附属第二医院
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Cgas , Bax siRNA
原文引用：For Cgas or Bax KD, LLC or MLO-Y4 cells were transfected with Cgas small interfering RNA (siRNA), Bax siRNA, or negative control siRNA (Genomeditech Co. Ltd.) relatively using Lipofectamine 3000 transfection reagents.
试剂产品篇
20、金属蛋白纳米颗粒支架通过金属增强的STING 激活共同递送抗原和佐剂，用于预防病毒感染的疫苗接种
文献标题：A metalloprotein nanoparticle scaffold co-delivers antigens and adjuvants with metal-enhanced STING activation for vaccination preventing viral infections
影响因子：17.4
发表期刊：Nano Today
发表单位：天津大学
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荧光素底物
原文引用：Then 200 μL luciferase substrate (Genomeditech) was added to measure luciferase activity.
21、NKD2 控制成骨细胞和破骨细胞的分化并改善卵巢切除小鼠的骨质流失
文献标题：Naked cuticle homolog 2 controls the differentiation of osteoblasts and osteoclasts and ameliorates bone loss in ovariectomized mice
影响因子：6.8
发表期刊：Genes & Diseases
发表单位：天津医科大学基础医学院
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慢病毒包装试剂盒
原文引用：The packaging of NKD2 or control vector lentivirus was performed by transfecting the plasmids into 293T cells using a lentiviral packaging kit (Genomeditech, Shanghai, China）
22、胆汁酸通过NLRP3炎症小体途径诱导肝纤维化及FXR抑制NLRP3激活的机制
文献标题：Bile acids induce liver fibrosis through the NLRP3 inflammasome pathway and the mechanism of FXR inhibition of NLRP3 activation
影响因子：6.6
发表期刊：Hepatology International
发表单位：贵州医科大学附属医院
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荧光素酶报告基因检测试剂盒
原文引用：Twenty-four hours after transfection, the luciferase activity of firefly and Renilla was measured with a luciferase reporter assay kit (Genomeditech, Shanghai, China)
23、RUNX1靶向AKT3促进LPS诱导的ARDS中肺泡高凝和纤溶抑制
文献标题：RUNX1 targeting AKT3 promotes alveolar hypercoagulation and fibrinolytic inhibition in LPS induced ARDS
影响因子：5.8
发表期刊：Respiratory Research
发表单位：贵州医科大学附属医院
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HG转染试剂和报告基因
原文引用：Next day, cells were co-transfected with the control pcDNA3.1 empty vector (EV, 250 ng) or the wild-type RUNX1 plasmid (250 ng)-based frefy luciferase reporter plus the renilla luciferase control vector pRL-TK (25 ng) using the HG transgene reagent (genomeditec, TG-10012-S). cell lysates were prepared and firely/renilla luciferase values were quantifed using Firely Luciferase Assay (Genomeditech).
