最新！二季度客户文献精选集锦来啦
2024年第二季度共收集引用Genomeditech Co.,Ltd. (Shangha,China)文章共计144篇，影响因子(IF)超880分。
其中包括Journal of Hepatology、Cancer Communications、Bioactive Materials、Nature communications、Advanced Science、Cell Death and Differentiation、Cell Discovery等影响因子10+以上的文章共30篇。
文章所涵盖的服务包含过表达/干扰质粒构建、慢病毒、腺相关病毒AAV、CRISPR/Cas9基因敲除、细胞株构建、荧光素酶报告基因等，此外还有荧光素底物（如钾盐），脂质体转染试剂，慢病毒包装和浓缩试剂盒，siRNA等常用科研类试剂。
本篇精选了二季度新发的部分高质量文章，让我们一起追踪最新的医学研究进展！
慢病毒 & 细胞系列
1、EMC10 以异构体特异性方式调节肝脏 ER 应激和脂肪变性
文献标题：EMC10 modulates hepatic ER stress and steatosis in an isoform-specific mann
影响因子：26.8
发表期刊：Journal of Hepatology
发表单位：复旦大学王宣春团队
摘要
【背景介绍】内质网 (ER) 膜蛋白复合物亚基 10 (EMC10) 与肥胖有关。作者研究了 EMC10 的两种同工型，包括分泌型同工型(scEMC10)和 ER 膜结合同工型(mEMC10)，在代谢功能障碍相关的脂肪肝病 (MASLD) 中的作用。
【研究方法】采用多种脂肪变性小鼠模型和 HepG2 细胞来研究 EMC10 在肝脏 PERK-eIF2α-ATF4 信号传导和肝脂肪变性调节中的作用。探讨scEMC10中和抗体对小鼠肝脂肪变性的治疗作用。
【研究结果】scEMC10 促进肝脏 PERK-eIF2α-ATF4信号传导的激活，而mEMC10 抑制。基因敲除加剧了小鼠的肝脏 ER 应激和脂肪变性，而mEMC10的肝脏过度表达则受到缺乏蛋氨酸和胆碱的饮食或衣霉素的挑战，这突显了mEMC10通过调节肝脏 ER 应激在 MASLD 中的直接抑制作用。scEMC10 的过度表达促进了脂肪肝小鼠的肝脂肪变性，而循环 scEMC10 的中和则阻止了其发生，这表明scEMC10在MASLD发展中的作用。
这些发现证明了 EMC10 在 MASLD 发病机制中具有新的异构体特异性作用，并通过基于抗体的中和确定了分泌的异构体作为 MASLD 的易处理治疗靶点。
吉满助力
pLEX-mEMC10过表达慢病毒
2、头颈鳞状细胞癌的代谢景观揭示了免疫逃逸中新的犬尿氨酸/SIGLEC-15轴
文献标题：Metabolic landscape of head and neck squamous cell carcinoma informs a novel kynurenine/Siglec-15 axis in immune escape
影响因子：20.09
发表期刊：Cancer Communications
发表单位：上海交通大学医学院附属第九人民医院
吉满助力
SIGLEC15过表达慢病毒
原文引用：Mouse SIGLEC15 cDNA was cloned into the PGMLV-CMV-MCS-EF1-ZsGreen1-T2A-Puro vector (Genomeditech, Shanghai, China).
3、ETV2调节PHD2-HIF-1α轴控制代谢重编程促进血管化骨再生
文献标题：ETV2 regulating PHD2-HIF-1α axis controls metabolism reprogramming promotes vascularized bone regeneration
影响因子：17.99
发表期刊：Bioactive Materials
发表单位：安徽医科大学
吉满助力
EGLN1(PHD2)过表达慢病毒；双荧光素酶报告基因
原文引用：EGLN1(PHD2)-encoding lentiviruses (Genomeditech, China) was constructed.
Target fragments were then inserted downstream of the firefly luciferase (FL) gene into the pGL3-basic luciferase reporter vector (Genomeditech) using seamless cloning
4、混合膜包覆纳米颗粒用于阿尔茨海默病的精确靶向和协同治疗
文献标题：Hybrid Membrane-Coated Nanoparticles for Precise Targeting and Synergistic Therapy in Alzheimer's Disease
影响因子：14.29
发表期刊：Advanced Science
发表单位：浙江大学
吉满助力
CCR2-RFP HEK293T稳转细胞株
原文引用：Stable CCR2-red fluorescent protein (RFP) transfected HEK293T and RFP transfected HEK293T cells were generated by Genomeditech, China.
5、靶向Rab7-Rilp介导的微脂肪自噬缓解糖尿病性心肌病中的脂质毒性
文献标题：Targeting Rab7-Rilp Mediated Microlipophagy Alleviates Lipid Toxicity in Diabetic Cardiomyopathy
影响因子：14.29
发表期刊：Advanced Science
发表单位：上海交通大学医学院附属第一人民医院
吉满助力
FLAG-Rab7 (WT), FLAG-Rab7 (S17A), FLAG-Rab7 (S72A), FLAG-Rab7 (Y183A) and HA-Rilp 过表达质粒；RFP-GFP-LC3自噬慢病毒；GST-Rilp or Rab7Y183A过表达慢病毒
原文引用：For transfection of siRNA, HL-1 cell were seeded in a six-well plate and transfected with siRilp (Genomeditech Co., Ltd. (Shanghai, China)) and si-control according to the manufacturer's instructions. For transfection of plasmid, HEK 293T was transfected with FLAG-Rab7 (WT), FLAG-Rab7 (S17A), FLAG-Rab7 (S72A), FLAG-Rab7 (Y183A) and HA-Rilp plasmids (Genomeditech Co., Ltd. (Shanghai, China)) and vector control according to the manufacture instructions. For transfection of RFP-GFP-LC3 lentivirus, HL-1 cells were seeded in a six-well plate or 96-well plates and transfected with RFP-GFP-LC3 (Genomeditech Co., Ltd. (Shanghai, China)) following the manufacture's protocol. For transfection of lentivirus, NMCMs or HL-1 cells were seed in 6 cm dishes (for immunoprecipitation), 6-well plates (for immunoblots) or 96-well plates (for immunoflurorences) before transfecting with GST-Rilp or Rab7Y183A lentivirus following protocols from Genomeditech Co., Ltd. (Shanghai, China).
6、PAX6通过增强MET/STAT5A介导的染色质可及性促进前列腺癌的神经内分泌表型
文献标题：PAX6 promotes neuroendocrine phenotypes of prostate cancer via enhancing MET/STAT5A-mediated chromatin accessibility
影响因子：11.39
发表期刊：Journal of Experimental & Clinical Cancer Research
发表单位：上海交通大学医学院
吉满助力
human PAX6 shRNA慢病毒
原文引用：A human PAX6 lentiviral expression construct containing a puromycin resistance gene was purchased from Genomeditech PAX6 short hairpin RNA (shRNA) expression constructs were purchased from Genomeditech.
7、伴随的 NAFLD 通过 CXCL5/CXCR2 招募结直肠癌中的 MDSC，促进肝转移和 PD-1 难治性
文献标题：Concomitant NAFLD Facilitates Liver Metastases and PD-1-Refractory by Recruiting MDSCs via CXCL5/CXCR2 in Colorectal Cancer
影响因子：7.1
发表期刊：Cellular and Molecular Gastroenterology and Hepatology
发表单位：山东省肿瘤医院
吉满助力
CMV-Luciferase-PGK-NEO活体成像慢病毒
原文引用：CMV-Luciferase-PGK-NEO lentivirus was purchased from Genomeditech and transfected into MC38 cells to make the cells fluorescent.
假病毒系列
8、通过分层介孔金属有机框架佐剂增强 STING 激活来增强体液和细胞免疫
文献标题：Boosting humoral and cellular immunity with enhanced STING activation by hierarchical mesoporous metal-organic framework adjuvants
影响因子：10.50
发表期刊：Journal of Controlled Release
发表单位：上海交通大学医学院
吉满助力
SARS-CoV-2 Spike (B.1.617.2) 假病毒
原文引用：The SARS-CoV-2 Spike (B.1.617.2) pseudotyped virus (GFP-luciferase) and GMOne-Step Luciferase Reporter Gene Assay Kit were purchased from Genomeditech Co. Ltd..
CRISPR/Cas9系列
9、源自 Akkermansia muciniphila 的十五烷酸通过调节糖酵解增强奥沙利铂对胃癌的敏感性
文献标题：Akkermansia muciniphila-derived pentadecanoic acid enhances oxaliplatin sensitivity in gastric cancer by modulating glycolysis
影响因子：9.1
发表期刊：Pharmacological Research
发表单位：解放军总医院
吉满助力
FUBP1敲除单克隆
原文引用：The CRISPR/Cas9 system was used to construct FUBP1-KO GC cell lines. The FUBP1 sgRNA was designed and synthesized by Genomeditech (Shanghai, China) For RALY-overexpression cells, full-length cDNA of mouse RALY was cloned and inserted into the PGMLV-CMV-MCS-Puro plasmid (Genomeditech, Shanghai, China).
10、MAP4K4通过促进Drp1的S-亚硝基化修饰加剧糖尿病患者的心脏微血管损伤
文献标题：MAP4K4 exacerbates cardiac microvascular injury in diabetes by facilitating S-nitrosylation modification of Drp1
影响因子：8.49
发表期刊：Cardiovascular Diabetology
发表单位：南京医科大学
吉满助力
Single-guide RNAs (sgRNAs)
原文引用：Genomic mutations were introduced into cells using the CRISPR–Cas9 system. Single-guide RNAs (sgRNAs) were designed by Genomeditech (China, Shanghai) to target the genomic area adjacent to mutation sites in C505 or C644.
腺相关病毒系列
11、腰椎不稳定重塑软骨终板，通过 Hippo-CCL3 信号招募破骨细胞诱导椎间盘退变
文献标题：Lumbar instability remodels cartilage endplate to induce intervertebral disc degeneration by recruiting osteoclasts via Hippo-CCL3 signaling
影响因子：14.29
发表期刊：Bone Research
发表单位：苏州大学附属第一医院
吉满助力
AAV5-Yap1 过表达AAV
原文引用：AAV5-Yap1 was purchased from Genomeditech.
12、LPS 诱导的单羧酸转运蛋白 1 抑制促进乳酸积累，引发上皮间质转化和肺纤维化
文献标题：LPS-induced monocarboxylate transporter-1 inhibition facilitates lactate accumulation triggering epithelial-mesenchymal transformation and pulmonary fibrosis
影响因子：6.2
发表期刊：Cellular and Molecular Life Sciences
发表单位：上海交通大学医学院附属仁济医院
吉满助力
MCT1过表达AAV5；抗生素嘌呤霉素；MCT1 siRNA
原文引用：In order to establish an alveolar epithelial cells specific MCT1-overexpressing (MCT1OE) mouse model, intratracheal administration of 40 μl MCT1OE adeno-associated virus (AAV) mixture (AAV concentration: 5 × 1012 VG/ml), purchased from Genomeditech (Shanghai, China)
After 2 days, stable-transfected cells were selected with 10 µg/mL of puromycin (Genomeditech, China).
Mouse MCT1 small interfering RNA (siRNA) was purchased from Genomeditech (Shanghai, China). MCT1 siRNA
质粒系列
13、线粒体基因组转移驱动邻近结肠上皮细胞的代谢重编程，促进 TGFβ1 介导的肿瘤进展
文献标题：Mitochondrial genome transfer drives metabolic reprogramming in adjacent colonic epithelial cells promoting TGFβ1-mediated tumor progression
影响因子：14.7
发表期刊：Nature Communications
发表单位：上海交通大学医学院附属第一人民医院
吉满助力
TFAM, RelA, IκBα, and TGFβ1 shRNA慢病毒质粒
原文引用：Non-target control shRNA (shNT) and shRNA targeting TFAM, RelA, IκBα, and TGFβ1 were cloned into the pGMLV-hU6 lentiviral vector (Genomeditech, Shanghai, China).
14、CCL9/CCR1 轴驱动的趋化纳米囊泡通过捕获 TGF-β 来减弱 SMAD4 缺陷型结直肠癌的转移
文献标题：CCL9/CCR1 axis-driven chemotactic nanovesicles for attenuating metastasis of SMAD4-deficient colorectal cancer by trapping TGF-β
影响因子：14.70
发表期刊：Acta Pharmaceutica Sinica B
发表单位：华中科技大学同济医学院
吉满助力
CCR1 or TGFBR2过表达慢病毒质粒
原文引用：MC38 stable cell lines were constructed by lentivirus. Plasmids encoding CCR1 or TGFBR2 (pLenti-GFP/puro-CCR1 and pLenti-RFP/puro-TGFBR2) were purchased from Genomeditech (Shanghai, China).
15、MEF2D在IL-13刺激下直接诱导H1X促进胃癌细胞肝转移
文献标题：MEF2D facilitates liver metastasis of gastric cancer cells through directly inducing H1X under IL-13 stimulation
影响因子：9.10
发表期刊：Cancer Letters
发表单位：浙江大学医学院附属第一医院
吉满助力
H1X and IL13RA1干扰和过表达质粒；
MEF2D过表达质粒和报告基因质粒
原文引用：The gene knockdown and overexpression plasmids of H1X and IL13RA1 were constructed by Genomeditech Co., Ltd. (Shanghai, China)
MEF2D-overexpressed plasmid and Luciferase Reporter plasmids were constructed by Genomeditech Co.,Ltd (Shanghai, China).
siRNA系列
16、骨细胞线粒体调节经皮层血管的血管生成
文献标题：Osteocyte mitochondria regulate angiogenesis of transcortical vessels
影响因子：14.70
发表期刊：Nature Communications
发表单位：上海交通大学
吉满助力
Mouse Slit3、Notch3、Notch4、Vegfc siRNA套餐
原文引用：To generate VegfcKD-MLO-Y4, Slit3KD-MLO-Y4, Notch3KD-MLO-Y4,Notch4KD-MLO-Y4 cells, cells were plated on 12 well plate, and incubated in 1% lipofectamine (Invitrogen, Cat. L3000015) and 50 nM siRNA (Genomeditech, Shanhai, China) in opti-MEM for 6 hours (the detailed siRNA sequences are listed in Supplementary Table 2c)
17、仿生水凝胶介导的机械免疫代谢疗法，可抑制ccRCC手术后复发
文献标题：Biomimetic Hydrogel-Mediated Mechano-Immunometabolic Therapy for Inhibition of ccRCC Recurrence After Surgery
影响因子：14.29
发表期刊：Advanced Science
发表单位：同济大学医学院 上海市第十人民医
吉满助力
IDO1 siRNA
原文引用：The siIDO1 (sense strand 5′-GCAAUAGUAGAUACUUACA-3′), the negative control siRNA (a scrambled sequence consisting of the sense strand 5′-UUCUCCGAACGUGUCACGUTT-3′) and the Cy3-siRNA were synthesized by Genomeditech Biotechnology Co., Ltd anti-GAPDH antibody (Genomeditech, dilution 1:1000)
试剂产品系列
18、苯并[a]芘的暴露诱导肝癌外泌体-环状RNA激活肺成纤维细胞并引发器官转移
文献标题：Exposure of benzo[a]pyrene induces HCC exosome-circular RNA to activate lung fibroblasts and trigger organotropic metastasis
影响因子：20.09
发表期刊：Cancer Communications
发表单位：上海交通大学医学院公共卫生学院
吉满助力
荧光素底物，钾盐
原文引用：intraperitoneal injection of 200 μL of D-luciferin (100 mg/kg, Genomeditech, Shanghai, China) .
19、通过分层介孔金属有机框架佐剂增强 STING 激活来增强体液和细胞免疫
文献标题：Boosting humoral and cellular immunity with enhanced STING activation by hierarchical mesoporous metal-organic framework adjuvants
影响因子：10.50
发表期刊：Journal of Controlled Release
发表单位：上海交通大学医学院
吉满助力
GMOne-Step 荧光素酶报告基因检测试剂盒
原文引用：The SARS-CoV-2 Spike (B.1.617.2) pseudotyped virus (GFP-luciferase) and GMOne-Step Luciferase Reporter Gene Assay Kit were purchased from Genomeditech Co. Ltd.
20、RBBP6 通过 CPSF3 依赖性替代多腺苷酸化维持胶质母细胞瘤干细胞
文献标题：RBBP6 maintains glioblastoma stem cells through CPSF3-dependent alternative polyadenylation
影响因子：12.99
发表期刊：Cell Discovery
发表单位：浙江大学生命科学学院
吉满助力
慢病毒浓缩试剂盒
原文引用：lentivirus concentration kit (Genomeditech, Cat#GM-040801-100)
21、整合素αVβ3拮抗剂-c(RGDyk)肽通过抑制成骨和血管生成来减轻后纵韧带骨化的进展
文献标题：Integrin αVβ3 antagonist-c(RGDyk) peptide attenuates the progression of ossification of the posterior longitudinal ligament by inhibiting osteogenesis and angiogenesis
影响因子：5.9998
发表期刊：Molecular Medicine
发表单位：海军军医大学（第二军医大学）长征医院
吉满助力
Integrin αV and Integrin β3 siRNAs和GMTrans 脂质体转染试剂
原文引用：The siRNAs targeting Integrin αV and Integrin β3 were designed and synthesized by Genomeditech Cor (Shanghai, China). LF transfection during osteoblastogenesis in vitro was performed on the first day after osteogenic differentiation induction. The siRNA and GMTrans Liposomal Transfection Reagent (Genomeditech, Shanghai, China)
