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慢病毒( Lentivirus )载体是以 HIV-1 (人类免疫缺陷1型病毒)为基础发展起来的基因治疗载体, 属于逆转录病毒科,为RNA病毒。区别于一般的逆转录病毒载体,它对分裂细胞和非分裂细胞均具有感染能力。该载体可以将外源基因有效地整合到宿主染色体上,从而达到持久性表达。目前慢病毒也被广泛地应用于表达 RNAi 的研究中。由于体外合成 siRNA 对基因表达的抑制作用通常是短暂的,因而使其应用受到较大的限制。基于慢病毒载体设计的shRNA , 转移到细胞内转录 siRNA 的策略,不但使有效转染的细胞种类增加,而且长期稳定抑制目的基因在细胞中表达。在感染能力方面可有效地感染神经元细胞、肝细胞、心肌细胞、肿瘤细胞、内皮细胞、干细胞等多种类型细胞,从而达到良好的基因治疗效果,在美国已经开展了临床研究,效果非常理想,因此具有广阔的应用前景。


                                                 过表达慢病毒包装       RNAi慢病毒包装

                                                                 miRNA慢病毒包装        稳定细胞株构建服务



  • 非常难转染的细胞,例如神经元细胞、原代细胞、干细胞、肿瘤细胞等,针对这类细胞慢病毒载体的转染效率接近100%;
  • 构建稳定表达或者沉默特定基因的单克隆细胞株;
  • 活体动物模型(in vivo)的基因操作;
  • 调控表达,经过特定的元件修饰的慢病毒表达载体,可实现只有在特定的外源或内源物质的作用下才能产生表达,从而调控目的基因的定时、定量表达。




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Clin Cancer Res (2017). [IF=9.619].Numb-/low enriches a castration resistant prostate cancer cell subpopulation associated with enhanced Notch and Hedgehog signaling.

Cancer Research(2017). [IF 8.556].WNT/β-catenin directs self-renewal symmetric cell division of hTERThigh prostate cancer stem cells.

Oncogene(2017). [IF 7.932].MiR-21 is required for anti-tumor immune response in mice: an implication for its bi-directional roles.

ACS Appl Mater Inter (2017). [IF7.145]. Osteogenesis Catalyzed by Titanium-Supported Silver Nanoparticles.

Mol Ther (2017). [IF 6.688].Antitumoral Cascade-Targeting Ligand for IL-6 Receptor-Mediated Gene Delivery to Glioma.

Molecular Cancer(2017). [IF=6.024]. Long noncoding RNA MRCCAT1 promotes metastasis of clear cell renal cell carcinoma via inhibiting NPR3 and activating p38-MAPK signaling.

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Mol Carcinogen(2017). [IF 4.722].Proteomic profiling of isogenic primary and metastatic medulloblastoma cell lines reveals differential expression of key metastatic factors.

Mol Carcinogen (2017). [IF 4.185].PTENP1 inhibits the growth of esophageal squamous cell carcinoma by regulating SOCS6 expression and correlates with disease prognosis.

J Cell Physiol(2017). [IF4.155]. Transcription factor EGR1 promotes differentiation of bovine skeletal muscle satellite cells by regulating MyoG gene expression.

Front in Physiol(2017). [IF 4.031].Lysophosphatidic Acid Is Associated with Atherosclerotic Plaque Instability by Regulating NF-κB Dependent Matrix Metalloproteinase-9 Expression via LPA2 in Macrophages. 

Stem Cell Transl Med(2017). [IF=4]. Angiogenic Potential of Human Bone Marrow-Derived Mesenchymal Stem Cells in Chondrocyte Brick-Enriched Constructs Promoted Stable Regeneration of Craniofacial Cartilage.

J of Funct Foods(2017). [IF 3.973].Rapid modulation of lipid metabolism in C57BL/6J mice induced by eicosapentaenoic acid-enriched phospholipid from Cucumaria frondosa.

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Toxicol Lett(2017). [IF 3.522].DJ-1 regulating PI3K-Nrf2 signaling plays a significant role in bibenzyl compound 20C-mediated neuroprotection against rotenone-induced oxidative insult.

Invest Ophth Vis Sci(2017). [IF 3.427].Glucocorticoid-Induced Leucine Zipper Suppresses ICAM-1 and MCP-1 Expression by Dephosphorylation of NF-κB p65 in Retinal Endothelial Cells.

Invest Ophth Vis Sci(2017). [IF 3.427].Glucocorticoid-Induced Leucine Zipper Protects the Retina From Light-Induced Retinal Degeneration by Inducing Bcl-xL in Rats.

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RSC Advances(2017). [IF 3.289].Overcoming the blood–brain barrier for glioma-targeted therapy based on an interleukin-6 receptor-mediated micelle system. 

Int J Mol Sci(2017). [IF 3.257].The Essential Role of Pin1 via NF-κB Signaling in Vascular Inflammation and Atherosclerosis in ApoE−/− Mice.

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Am J Transl Res(2017). [IF 3.146].RNA helicase DHX9 may be a therapeutic target in lung cancer and inhibited by enoxacin.                  

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J Cell Biochem(2017). [IF=3.085]. miR-31 Regulates Spermatogonial Stem Cells Meiosis via Targeting Stra8.

J Cell Biochem(2017). [IF=3.085].CREPT and p15RS regulate cell proliferation and cycling in chicken DF-1 cells through the Wnt/β-catenin pathway.

J Cell Biochem (2017). [IF=3.085].MAPK8 Regulates Chicken Male Germ Cell Differentiation Through JNK Signaling Pathway.

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Free Radical Res(2017). [IF 2.949].Astragaloside IV synergizes with ferulic acid to suppress hepatic stellate cells activation in vitro. 

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J Clin Pathol(2017). [IF 2.912].Mortalin promotes cell proliferation and epithelial mesenchymal transition of intrahepatic cholangiocarcinoma cells in vitro.      

Am J Transl Res (2017). [IF=2.829].RNA helicase DHX9 may be a therapeutic target in lung cancer and inhibited by enoxacin.

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Oncol Rep (2017). [IF 2.486].miR-30a radiosensitizes non-small cell lung cancer by targeting ATF1 that is involved in the phosphorylation of ATM. 

Oncol Rep(2017). [IF 2.486].Silencing of ANXA3 expression by RNA interference inhibits the proliferation and invasion of breast cancer cells. 

Oncol Rep(2017). [IF2.486]. Capn4 promotes epithelial-mesenchymal transition in human melanoma cells through activation of the Wnt/β-catenin pathway. 

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Curr Eye Res(2017). [IF 2.025].Overexpression of ALK5 Induces Human Tenon's Capsule Fibroblasts Transdifferentiation and Fibrosis In Vitro. 

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Oncol Res(2017). [IF=1.740]. Overexpression of PER3 Inhibits Self-Renewal Capability and Chemoresistance of Colorectal Cancer Stem-Like Cells via Inhibition of Notch and β-Catenin Signaling.

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Anim Reprod Sci(2017). [IF 1.377].Hedgehog-Gli1 signaling regelates differentiation of chicken (Gallus gallus) embryonic stem cells to male germ cells. 

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