来了来了！四季度客户文献精选集锦来啦
四季度共收集引用Genomeditech Co.,Ltd. (Shangha,China)文章共计96篇，影响因子(IF)超500分。其中包括Cancer Cell以及Nucleic Acids Research，Advanced Science，Nature Communications影响因子15+以上的文章8篇。
文章所涵盖的产品和服务包含常规质粒构建、慢病毒、腺相关病毒、细胞株构建、活体成像、荧光素酶报告基因，还包含了自噬，circRNA，CRISPR/Cas9技术等。
本篇精选了四季度新发的部分高质量文章，让我们一起追踪最新科研进展！
细胞产品篇
1、多组学综合图谱解析转移性肝癌的时空演变
文献标题：Integrated multi-omics profiling to dissect the spatiotemporal evolution of metastatic hepatocellular carcinoma
影响因子：50.3
发表期刊：Cancer Cell
发表单位：复旦大学附属中山医院
摘要：目前肝癌转移肿瘤的基因突变和分子表达全景图谱仍极度缺乏，肝癌在转移过程中的克隆选择等演化规律尚不清晰，导致肝癌转移病灶的临床诊疗仍然缺乏足够的理论指导。在这里，作者对182例HCC患者的257个原发性和176个转移灶进行了多组分析。原发性和转移性HCC的基因组差异很高，早期播散很普遍。在转移瘤中观察到的显著的肿瘤内新抗原异质性与T细胞反应性降低有关，这是由于新抗原递呈中断所致。没有Wnt突变的亚克隆比有Wnt突变的亚克隆显示出更强的转移选择性优势，并以微环境中丰富的活化成纤维细胞为特征，有利于前转移表型。最后，没有Wnt突变的转移瘤表现出更高的免疫抑制B细胞富集，通过HLA-E:CD94-NKG2A检查点轴介导CD8+ T细胞的末端衰竭。总的来说，作者的结果提供了一个复杂的转移进化过程的多维解剖。
吉满助力
AXIN1敲除单克隆细胞系：AXIN1-knockout MHCC97H cell line
干扰慢病毒：PGMLV-hU6-sh-HIF1a-CMV-Blasticidin-WPRE
过表达慢病毒：PGMLV-CMV-HIF1a-33Flag-PGK-Blasticidin
2、Claudin18.2/4–1BB 双特异性抗体- 792 Givastomig 在 1L/2L 胃癌治疗中发挥旁观者肿瘤杀伤和协同抗肿瘤活性
文献标题：792 Givastomig, a novel Claudin18.2/4–1BB bispecific antibody, exerts bystander tumor-killing and synergistic anti-tumor activity with therapeutics in 1L/2L treatment for gastric cancer
影响因子：10.9
发表期刊：Journal for Immunotherapy of Cancer
发表单位：天境生物制药
吉满助力
CLDN18.2过表达细胞株
原文引用：The CLDN18.2 expression in formalin-fixed,paraffin-embedded(FFPE) tumors of three human gastric cancer cell lines (MKN-45,MKN-45#14,and MKN-45#18; obtained from Genomeditech)
3、一种新型介孔二氧化硅纳米系统为治疗SARS-CoV-2感染提供了新的可能
文献标题：A coronavirus-mimic mesoporous silica nanosystem enables efficient targeted delivery of siRNA for anti-SARS-CoV‐2
影响因子：8.3
发表期刊：Applied Materials Today
发表单位：中国科学院上海硅酸盐研究所
吉满助力
ACE2 OE-293T过表达细胞株
原文引用：In addition, the RdRp gene of the SARS-CoV-2 genome (MT039890) was stably transfected into the ACE2 OE-293T cells by a lentivirus infection method (Genomeditech)
Luciferase产品篇
4、肿瘤内真菌群落通过髓源性抑制细胞促进肺癌进展
文献标题：Histone lactylation-boosted ALKBH3 potentiates tumor progression and diminished promyelocytic leukemia protein nuclear condensates by m1A demethylation of SP100A
影响因子：19.2
发表期刊：Nucleic Acids Research
发表单位：上海交通大学医学院附属第九人民医院
吉满助力
ALKBH3/SP100A过表达和干扰慢病毒质粒
原文引用：The PCMLW-CMV-MCS-EF1-ZsGreen1-T2A-Puro,CMLV-hU6-MCS-CMV-ZsGreen1-PCK-Puro WPRE,ZV502 and ZV102 vectors were used in our study. ALKBH3 shRNAs，SP100A shRNA,YTHDF1 shRNA and their corresponding verified negative control sequences were amplified by PCR and inserted into the PCMLV-hU6-MCS-CMV-ZsGreen1-PGK-Puro-WPRE vectors. The SP100A ORF was generated by PCR and cloned into the PCMLV-CMV-MCS-EF1-ZsGreen1-T2A-Puro vector.
吉满助力
m1A甲基化；SP100A-5UTR报告基因质粒
原文引用：The DNA fragments of the SP100A-5UTR containing the wild-type m1A motifs and mutant motifs (potential m A was replaced by T) were synthesized and inserted upstream of firefly luciferase of the pmirCLO vector.
5、牛磺酸抑制前列腺癌肿瘤细胞和肿瘤相关巨噬细胞之间的串扰介导的铁死亡
文献标题：Taurine Inhibits Ferroptosis Mediated by the Crosstalk between Tumor Cells and Tumor-Associated Macrophages in Prostate Cancer
影响因子：15.1
发表期刊：Advanced Science
发表单位：上海交通大学医学院附属第九人民医院
吉满助力
lats1 mRNA 3′-UTR报告基因质粒
原文引用：For the dual luciferase assay, wild type (WT) or mutant (MT) putative miR-181a-5p binding site sequences on lats1 mRNA 3′-UTR region were purchased from Genomeditech
质粒和慢病毒产品篇
6、BPTF 通过表观遗传调控 C-MYC/PLCG1/Perk 轴来驱动胃癌对 EGFR 抑制剂的耐药性
文献标题：BPTF Drives Gastric Cancer Resistance to EGFR Inhibitor by Epigenetically Regulating the C-MYC/PLCG1/Perk Axis
影响因子：15.1
发表期刊：Advanced Science
发表单位：上海交通大学医学院附属瑞金医院
吉满助力
PLCG1过表达和siRNA
原文引用：Overexpression and Knockdown of PLCG1 and c-MYC Genes: Control and PLCG1/c-MYC siRNAs (PLCG1, GAGTTTGTGGGTCAGTCTG, cMYC, GCTTCACCAACAGGAACTA) were purchased from Genomeditech(Shanghai, China)
7、锰通过靶向 T 淋巴细胞极化增强硅羟基磷灰石纳米线的成骨作用
文献标题：Manganese Enhances the Osteogenic Effect of Silicon-Hydroxyapatite Nanowires by Targeting T Lymphocyte Polarization
影响因子：15.1
发表期刊：Advanced Science
发表单位：上海交通大学医学院附属第九人民医院
吉满助力
小鼠Sod2干扰慢病毒
原文引用：The MnSOD shRNA lentivirus was constructed by Genomeditech(Shanghai, China).
8、富马酸二甲酯（DMF）在改善双侧海绵体神经损伤（BCNI）大鼠勃起功能方面的治疗潜力
文献标题：Dimethyl fumarate ameliorates erectile dysfunction in bilateral cavernous nerve injury rats by inhibiting oxidative stress and NLRP3 inflammasome-mediated pyroptosis of nerve via activation of Nrf2/HO-1 signaling pathway
影响因子：11.4
发表期刊：Redox Biology
发表单位：华中科技大学同济医学院附属同济医院
吉满助力
大鼠Nfe2l2、Ho-1干扰慢病毒质粒
原文引用：The lentiviral vectors were generated and the competent cells containing target plasmids were constructed by Genomeditech
9、非ACE2依赖SARS-CoV-2病毒通过单核细胞表面GRP78感染
文献标题：ACE2-independent SARS-CoV-2 virus entry through cell surface GRP78 on monocytes -evidence from a translational clinical and experimental approach
影响因子：11.1
发表期刊：EBioMedicine
发表单位：华中科技大学同济医学院
吉满助力
GRP78 过表达质粒和慢病毒；HG转染试剂
原文引用：To establish overexpression of GRP78 in THP-1 cells, human HSPA5 gene segment was inserted into PGMLV-CMV-MCS-EF1-mScarlet-T2A-Puro plasmid (Genomeditech, Shanghai, China). hGRP78 over-expressed plasmid or mock control plasmid were co-transfected with lentivirus-packaging plasmid (Genomeditech, Shanghai, China) into HEK293T cells using HG transgene reagent (Genomeditech, Shanghai, China) according to the manufacturer's protocol.
腺相关病毒产品篇
10、一种新型酞嗪酮衍生物作为衣壳组装调节剂抑制乙型肝炎病毒表达
文献标题：A novel phthalazinone derivative as a capsid assembly modulator inhibits hepatitis B virus expression
影响因子：7.6
发表期刊：Antiviral Research
发表单位：中国科学院上海药物研究所
吉满助力
HBV（AYW）腺相关病毒
原文引用：Adeno-associated virus (capsid serotype AAV8) expressing a 1.1-mer overlength HBV genome (serotype ayw) was purchased from Genomeditech (Shanghai, China)
11、METTL3通过促进TRAF6依赖性线粒体ROS产生导致MI后交感神经亢进
文献标题：m6A methyltransferase METTL3 contributes to sympathetic hyperactivity post-MI via promoting TRAF6-dependent mitochondrial ROS production
影响因子：7.4
发表期刊：Free Radical Biology and Medicine
发表单位：山东第一医科大学附属第一医院
吉满助力
ACE2 OE-293T过表达细胞株
原文引用：In addition, the RdRp gene of the SARS-CoV-2 genome (MT039890) was stably transfected into the ACE2 OE-293T cells by a lentivirus infection method (Genomeditech)
现货产品篇
12、LncRNA INHEG 通过调节 rRNA 2'-O-甲基化促进胶质瘤干细胞的维持和致瘤性
文献标题：LncRNA INHEG promotes glioma stem cell maintenance and tumorigenicity through regulating rRNA 2’-O-methylation
影响因子：16.6
发表期刊：Nature Communications
发表单位：中国科学院生物物理研究所
吉满助力
慢病毒浓缩试剂盒
原文引用：Lentiviral particles were collected 48 h after media change and concentrated using the lentivirus concentrated kit (Genomeditech) according to the manufacturer’s instructions
13、一种抗体调理作用的免疫代谢 NP (immeNPs)，用于肿瘤浸润单核吞噬的靶向双重干预
文献标题：Reshaping Intratumoral Mononuclear Phagocytes with Antibody-Opsonized Immunometabolic Nanoparticles
影响因子：15.1004
发表期刊：Advanced Science
发表单位：上海交通大学医学院
吉满助力
D-Luciferin(150μg−1,Genomeditech, GM040611) 荧光素钠盐
